They do need to be housed separately from now on, yes. No play time together at all– they’ll be fine separate. Rabbits do not really acknowledge the “family”– parents and siblings are still likely to fight and even mate with each other. It is very common that siblings will be fine to be together, but once hormones hit, which is happening for you now, that “baby bond” breaks. A baby bond is a temporary bond. A real bond (i.e. bonded pair of rabbits) occurs when 1) both rabbits are fixed and 2) they go through a bonding process to ensure they have established a safe and secure relationship dynamic.

Make sure you look into an exotic vet, not a dog/cat vet, to get your rabbits spayed.

I agree with Wick. Rabbit puberty, especially in girl rabbits, often manifests very suddenly, like the flipping of a switch.

Agree with the others. This is a common problem unfortunately, for some more info you can check out this pinned post:

“Help! Why are my rabbits fighting?!” By DanaNM

Yep, once they are spayed and have some time for their hormones to settle, you can start the bonding process. They can be housed side-by-side in the mean time though (just make sure they can’t nip noses through the fence). You should wait at least 3 weeks after spaying, but it can take longer for hormones to fully stabilize. Some people opt to wait 4 weeks or so, then try some sessions and see how things go. If things still seem really hormonal with them, you can give them more time.