Hmmm, are you set on having the three as a trio? There is a risk of the original bond breaking, but it was likely made a lot worse because Ruby wasn’t spayed.
I don’t have any trio experience, but once Ruby’s hormones have settled (she needs at least another 5 weeks), I think a first step would be to place her hutch near Mable and Peanut and see how they react. They might not be as hostile now that she is spayed. But yes, wait a bit more, as her hormones are still draining.
Sirius and Luna had this same problem with their pair: https://binkybunny.com/FORUM/tabid/54/aft/164686/Default.aspx
They chose to separate the pair until it was time to try to bond all three, as they also started fighting with a new bunny in the house.
People have different strategies for bonding trios. Some like to put all three buns together, other’s like to work with each pair combo first. I think a lot of it depends on how much bonding experience you have and what you feel comfortable with.
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The answers provided in this discussion are for general guideline purposes only. The information is not intended to diagnose or treat your pet. Seek the advice of your veterinarian or a qualified behaviorist.