How old is Rue?

It’s hard to say whats “right or wrong” really as it varies bunny to bunny. If she handled being picked up before, then it’s probably something you could work on again, in moderation. I think I would only hold her for a short time though. How did she act after you held her before this change? Did she hop away and thump of give foot flicks, or go hide? Did she accept food after?

And now, she is fighting it, is that right? This is where it can get risky. I’d only do it when sitting on the floor so she’s not got far to go if she jumps free. The floor being closer may help visually for her also.

As jg mentions, it varies. Rather than thinking of it as systematically, I say just read the mood. If she seems more willing to tolerate it, use that period of time, but if she’s not into it, hands off. Part of the trust for bunny handling is the bunny’s belief that the owner will not do it at times the rabbit doesn’t want them to (while eating, grooming, sleeping, etc.)