You definitely have to separate them immediately. It sounds like they might be young bunnies, which is why they were fine together at first, but now may be entering puberty. I’m assuming that they have not been spayed yet. It doesn’t matter if they are both female and if they were fine when housed together before. Maturing and puberty changes everything. You will end up with severe injuries or worse if you don’t separate them now. They can also come to hate each other and never want to be together if they fight and injure each other. Most vets will spay rabbits when they are 5 or 6 months old. Until then, they will have to be kept apart, not even let out to play and exercise together. After their spays and a few weeks have elapsed for them to heal and for their hormones to dissipate, you can start bonding sessions.
Here are 2 good articles on bonding:
http://rabbit.org/faq-bonding-multiple-rabbits/
https://binkybunny.com/BUNNYINFO/tabid/53/CategoryID/9/PID/940/Default.aspx